DNA extraction from formalin fixed franciscana tissues

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Dna Extraction from Formalin Fixed Franciscana Tissues

– The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169μg DNA/mg versus 0.808 ± 0.297μg DNA/mg). Similar results were obta...

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Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues

Formalin-fixed paraffin-embedded (FFPE) tissues are an important sample source for ret‐ rospective studies. Despite its ability to preserve proteins and cell morphology, formalin hinders molecular biology tests since it fragments and chemically modifies nucleic acids, especially RNA. Although several studies describe techniques that allow extracting nu‐ cleic acids from FFPE tissues, so far the...

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Improvement of DNA Extraction for Human Papillomavirus Genotyping from Formalin-Fixed Paraffin-Embedded Tissues

Mucosal human papillomaviruses (HPVs) play a crucial role in the development of cervical carcinoma, the third most common malignancy in women worldwide. These viruses are classified as high risk (HR) or low risk (LR), depending on their transforming ability. Formalinfixed paraffin-embedded (FFPE) tissues represent the most frequent form of tissue storage in pathology departments. These archival...

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Comparison of Methods for the Extraction of DNA from Formalin-Fixed, Paraffin-Embedded Archival Tissues

AIM Discussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues. METHODS DNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chlor...

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An efficient protocol for genomic DNA extraction from formalin-fixed paraffin-embedded tissues.

Formalin-fixed paraffin-embedded tissues (FFPET) represent the largest source of archival biological material available for genomic studies. In this work we present an advanced protocol for extraction of high quality DNA from FFPET that can be applied in several molecular studies. Although cat mammary tumours (CMT) are the third most frequent tumour in cats the recovery of significant number of...

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ژورنال

عنوان ژورنال: Latin American Journal of Aquatic Mammals

سال: 2002

ISSN: 2236-1057,1676-7497

DOI: 10.5597/lajam00016